Fig 2: Humoral immune responses and IFN‐γ and IL‐2 levels in guinea pigs immunized with LAV. A, Study design for VZV LAV immunization and schedules. All groups were inoculated twice at 2‐week intervals. Sera at pre‐injection (Day ‐5), 14 days, and 32 days after the first immunization were analyzed for total IgG (B), IgG1 (C), and IgG2 (D). IFN‐γ (E) and IL‐2 (F) levels in supernatants of cultured splenocytes after stimulation with LAV were measured by cytokine ELISA. Data are means ± SD. ELISA, enzyme‐linked immunosorbent assay; IFN‐γ, interferon‐γ; IL‐2, interleukin‐2; IgG, immunoglobulin G; LAV, live attenuated vaccine.** P < .01, *P < .05 compared with PBS immunization group G1